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oligo dt magnetic beads  (New England Biolabs)


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    New England Biolabs oligo dt magnetic beads
    Oligo Dt Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 917 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligo+dt+magnetic+beads/Oligo+dT25+Mag+Beads/pmc13171292-311-8-12
    Average 96 stars, based on 917 article reviews
    oligo dt magnetic beads - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown
    Article Snippet: 1 M DTT (Thermo Scientific, catalog number: R0861) 6. .. Oligo-dT magnetic beads (NEB, catalog number: S1419S) 7. .. 1% (w/v) methylene blue (Thermo Scientific, catalog number: 042771.AP) 8.

    Article Title: Neuroprotective mechanism of acupuncture against brain injury during delayed thrombolysis for acute ischemic stroke.
    Article Snippet: Journal of Integrative Medicine xxx (xxxx) xxx Contents lists available at ScienceDirect Journal of Integrati ve Medicine journal homepag e: www.jc imjournal .com/j im www.journals .e lsevier .com/journal -of - integrat ive-medic ine Original Research Article Neuroprotective mechanism of acupuncture against brain injury during delayed thrombolysis for acute ischemic stroke ⁎ Corresponding authors at: College of Acupuncture–Moxibustion and Tuina, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, Ch Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China.. E-mail addresses: xinchang@njucm.edu.cn (X.C.. Zhang), xgn66@njucm.edu.cn (G.X.

    Article Title: Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown
    Article Snippet: 1 M DTT (Thermo Scientific, catalog number: R0861) 6. .. Oligo-dT magnetic beads (NEB, catalog number: S1419S) 7. .. 1% (w/v) methylene blue (Thermo Scientific, catalog number: 042771.AP) 8.

    Article Title: Transcriptome Sequencing and Differential Analysis of Testes of 1-Year-Old and 3-Year-Old Kazakh Horses
    Article Snippet: Total RNA was extracted from testicular tissues using the TrizolTM Kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s standard protocol. .. RNA quality was strictly assessed through dual verification: (1) RNase-free agarose gel electrophoresis (1.5%) was used to visualize the integrity of 28S and 18S rRNA bands, with qualified samples showing clear, non-degraded bands without smearing; (2) an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) was employed to determine the RNA Integrity Number (RIN ≥ 8.0) and A260/A280 ratio (1.8–2.0), ensuring only high-quality RNA was used for subsequent experiments. mRNA was enriched from total RNA using oligo(dT) magnetic beads (NEB, Ipswich, MA, USA) that specifically bind the poly(A) tail of mRNA, followed by two rounds of purification to eliminate rRNA and other non-coding RNAs. .. The enriched mRNA was fragmented into 200–300 bp fragments using NEBNext First Strand Synthesis Reaction Buffer (5×) at 94 ◦C for 8 min. https://doi.org/10.3390/biology15010100 First-strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (NEB #M0253), and second-strand cDNA was generated using DNA Polymerase I (NEB #M0209) and RNase H (NEB #M0297).

    Article Title: A 50-tissue transcriptome atlas of the duck for gene network and functional analysis
    Article Snippet: Samples with RIN ≥ 6.5 and total yield ≥ 1 μg were used for library preparation with the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA). .. Polyadenylated mRNAs were enriched using Oligo(dT) magnetic beads (New England Biolabs) and fragmented using divalent cations under elevated temperature. ..

    Article Title: Transcriptome Sequencing and Differential Analysis of Testes of 1-Year-Old and 3-Year-Old Kazakh Horses
    Article Snippet: Total RNA was extracted from testicular tissues using the TrizolTM Kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s standard protocol. .. RNA quality was strictly assessed through dual verification: (1) RNase-free agarose gel electrophoresis (1.5%) was used to visualize the integrity of 28S and 18S rRNA bands, with qualified samples showing clear, non-degraded bands without smearing; (2) an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) was employed to determine the RNA Integrity Number (RIN ≥ 8.0) and A260/A280 ratio (1.8–2.0), ensuring only high-quality RNA was used for subsequent experiments. mRNA was enriched from total RNA using oligo(dT) magnetic beads (NEB, Ipswich, MA, USA) that specifically bind the poly(A) tail of mRNA, followed by two rounds of purification to eliminate rRNA and other non-coding RNAs. .. The enriched mRNA was fragmented into 200–300 bp fragments using NEBNext First Strand Synthesis Reaction Buffer (5×) at 94 °C for 8 min. First-strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (NEB #M0253), and second-strand cDNA was generated using DNA Polymerase I (NEB #M0209) and RNase H (NEB #M0297).

    Article Title: BNIP3 ‐Dependent Mitophagy Non‐Autonomously Regulates Systemic Aging via NF ‐ κB Suppression in Drosophila
    Article Snippet: The concentration and integrity of RNA concentration and integrity were assayed using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA), respectively. .. Poly(A) RNAs were isolated from total RNA using oligo‐dT magnetic beads (S1419S, New England Biolabs, Ipswich, MA). ..

    Article Title: An oral engineered cerium-peptide composite microsphere inhibits inflammatory bowel disease and reverses chronic fibrosis​​.
    Article Snippet: Bulk RNA-seq reveals differential gene expression in polarized RAW 264.7 macrophages Total RNA was isolated from RAW 264.7 macrophages using TRIzolTM reagent (Ambion, Cat. No. 15596018) according to the manufacturer’s instructions. .. Messenger RNA (mRNA) was purified from the total RNA using Oligo(dT) magnetic beads provided in the NEBNext® UltraTM II RNA Library Prep Kit for Illumina® (New England Biolabs Inc., Ipswich, MA, USA). ..

    Agarose Gel Electrophoresis:

    Article Title: Transcriptome Sequencing and Differential Analysis of Testes of 1-Year-Old and 3-Year-Old Kazakh Horses
    Article Snippet: Total RNA was extracted from testicular tissues using the TrizolTM Kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s standard protocol. .. RNA quality was strictly assessed through dual verification: (1) RNase-free agarose gel electrophoresis (1.5%) was used to visualize the integrity of 28S and 18S rRNA bands, with qualified samples showing clear, non-degraded bands without smearing; (2) an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) was employed to determine the RNA Integrity Number (RIN ≥ 8.0) and A260/A280 ratio (1.8–2.0), ensuring only high-quality RNA was used for subsequent experiments. mRNA was enriched from total RNA using oligo(dT) magnetic beads (NEB, Ipswich, MA, USA) that specifically bind the poly(A) tail of mRNA, followed by two rounds of purification to eliminate rRNA and other non-coding RNAs. .. The enriched mRNA was fragmented into 200–300 bp fragments using NEBNext First Strand Synthesis Reaction Buffer (5×) at 94 ◦C for 8 min. https://doi.org/10.3390/biology15010100 First-strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (NEB #M0253), and second-strand cDNA was generated using DNA Polymerase I (NEB #M0209) and RNase H (NEB #M0297).

    Article Title: Transcriptome Sequencing and Differential Analysis of Testes of 1-Year-Old and 3-Year-Old Kazakh Horses
    Article Snippet: Total RNA was extracted from testicular tissues using the TrizolTM Kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s standard protocol. .. RNA quality was strictly assessed through dual verification: (1) RNase-free agarose gel electrophoresis (1.5%) was used to visualize the integrity of 28S and 18S rRNA bands, with qualified samples showing clear, non-degraded bands without smearing; (2) an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) was employed to determine the RNA Integrity Number (RIN ≥ 8.0) and A260/A280 ratio (1.8–2.0), ensuring only high-quality RNA was used for subsequent experiments. mRNA was enriched from total RNA using oligo(dT) magnetic beads (NEB, Ipswich, MA, USA) that specifically bind the poly(A) tail of mRNA, followed by two rounds of purification to eliminate rRNA and other non-coding RNAs. .. The enriched mRNA was fragmented into 200–300 bp fragments using NEBNext First Strand Synthesis Reaction Buffer (5×) at 94 °C for 8 min. First-strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (NEB #M0253), and second-strand cDNA was generated using DNA Polymerase I (NEB #M0209) and RNase H (NEB #M0297).

    Purification:

    Article Title: Transcriptome Sequencing and Differential Analysis of Testes of 1-Year-Old and 3-Year-Old Kazakh Horses
    Article Snippet: Total RNA was extracted from testicular tissues using the TrizolTM Kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s standard protocol. .. RNA quality was strictly assessed through dual verification: (1) RNase-free agarose gel electrophoresis (1.5%) was used to visualize the integrity of 28S and 18S rRNA bands, with qualified samples showing clear, non-degraded bands without smearing; (2) an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) was employed to determine the RNA Integrity Number (RIN ≥ 8.0) and A260/A280 ratio (1.8–2.0), ensuring only high-quality RNA was used for subsequent experiments. mRNA was enriched from total RNA using oligo(dT) magnetic beads (NEB, Ipswich, MA, USA) that specifically bind the poly(A) tail of mRNA, followed by two rounds of purification to eliminate rRNA and other non-coding RNAs. .. The enriched mRNA was fragmented into 200–300 bp fragments using NEBNext First Strand Synthesis Reaction Buffer (5×) at 94 ◦C for 8 min. https://doi.org/10.3390/biology15010100 First-strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (NEB #M0253), and second-strand cDNA was generated using DNA Polymerase I (NEB #M0209) and RNase H (NEB #M0297).

    Article Title: Transcriptome Sequencing and Differential Analysis of Testes of 1-Year-Old and 3-Year-Old Kazakh Horses
    Article Snippet: Total RNA was extracted from testicular tissues using the TrizolTM Kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s standard protocol. .. RNA quality was strictly assessed through dual verification: (1) RNase-free agarose gel electrophoresis (1.5%) was used to visualize the integrity of 28S and 18S rRNA bands, with qualified samples showing clear, non-degraded bands without smearing; (2) an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) was employed to determine the RNA Integrity Number (RIN ≥ 8.0) and A260/A280 ratio (1.8–2.0), ensuring only high-quality RNA was used for subsequent experiments. mRNA was enriched from total RNA using oligo(dT) magnetic beads (NEB, Ipswich, MA, USA) that specifically bind the poly(A) tail of mRNA, followed by two rounds of purification to eliminate rRNA and other non-coding RNAs. .. The enriched mRNA was fragmented into 200–300 bp fragments using NEBNext First Strand Synthesis Reaction Buffer (5×) at 94 °C for 8 min. First-strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (NEB #M0253), and second-strand cDNA was generated using DNA Polymerase I (NEB #M0209) and RNase H (NEB #M0297).

    Article Title: An oral engineered cerium-peptide composite microsphere inhibits inflammatory bowel disease and reverses chronic fibrosis​​.
    Article Snippet: Bulk RNA-seq reveals differential gene expression in polarized RAW 264.7 macrophages Total RNA was isolated from RAW 264.7 macrophages using TRIzolTM reagent (Ambion, Cat. No. 15596018) according to the manufacturer’s instructions. .. Messenger RNA (mRNA) was purified from the total RNA using Oligo(dT) magnetic beads provided in the NEBNext® UltraTM II RNA Library Prep Kit for Illumina® (New England Biolabs Inc., Ipswich, MA, USA). ..

    Isolation:

    Article Title: BNIP3 ‐Dependent Mitophagy Non‐Autonomously Regulates Systemic Aging via NF ‐ κB Suppression in Drosophila
    Article Snippet: The concentration and integrity of RNA concentration and integrity were assayed using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA), respectively. .. Poly(A) RNAs were isolated from total RNA using oligo‐dT magnetic beads (S1419S, New England Biolabs, Ipswich, MA). ..



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